A new subtilisin-like proteinase from roots of the dandelion Taraxacum officinale Webb S. L.

نویسندگان

  • A M Bogacheva
  • G N Rudenskaya
  • A Preusser
  • I O Tchikileva
  • Y E Dunaevsky
  • B N Golovkin
  • V M Stepanov
چکیده

A serine proteinase from roots of Taraxacum officinale Webb S. L. was isolated by affinity chromatography and gel-filtration on Superose 6R using FPLC. The enzyme is a 67-kD glycoprotein containing 54% carbohydrate which we have named taraxalisin. The substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin B-chain is comparable with that of cucumisin from Cucumis melo and the subtilisin-like serine proteinase macluralisin from Maclura pomifera. The proteinase is inactivated by DFP and PMSF. Taraxalisin exhibits maximal activity at pH 8.0. The pH range for stability of the enzyme is narrow--6.0-9.0. The temperature optimum for the subtilisin-like activity is 40 degrees C. The N-terminal sequence of taraxalisin has 40% of its residues identical to those of subtilisin Carlsberg. Thus, the serine proteinase from dandelion roots is a member of the subtilisin family, which is evidently widespread in the plant kingdom.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of dandelion or a cover crop on mycorrhiza inoculum potential, soil aggregation and yield of maize

A field experiment was conducted to observe the influence of a cover crop (winter wheat, Triticum aestivum L.), and a perennial weed (dandelion, Taraxacum officinale Weber ex Wigg.), on vesicular-arbuscular mycorrhiza (VAM) inoculum potential, soil aggregation, and maize yield after one season. Mycorrhizal colonization of maize roots was higher following the autumn planting of either winter whe...

متن کامل

Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells.

Ethnotraditional use of plant-derived natural products plays a significant role in the discovery and development of potential medicinal agents. Plants of the genus Taraxacum, commonly known as dandelions, have a history of use in Chinese, Arabian and Native American traditional medicine, to treat a variety of diseases including cancer. To date, however, very few studies have been reported on th...

متن کامل

The biology of Canadian weeds. 117. Taraxacum officinale

] von Hinrichs, G. 1988. Control of powdery mildew on cultivated dandelion (Taraxacum officinale). Nachrichtenbl. Dtsch Pflanzenschutzdienst. (Braunsw.) 40(S): 116–120. von Hinrichs, G. 1989. Bekämpfung des echten Mehltaus an Kulturlöwenzahn. GB+GW 89(31): 1487–1490. [in German.] von Hofsten, C. G. 1954. Studies on the genus Taraxacum with special reference to the group Vulgaria DT in Scandinav...

متن کامل

Assessment of Heavy Metal Pollution of Medicinal Plants Taraxacum Officinale Web. Growing in the Kuznetsk Basin Areas Affected by Coal Production

We have studied the features of heavy metal accumulation in and have carried out a hygienic assessment of medicinal plants Taraxacum officinale Web. growing in various areas of the microrelief of the Kedrovskii coal mine rock dump. We have revealed a higher variability in the content of Cr, Mn, Zn and Fe mobile forms in the waste embryonic soils and have found that Cu and Zn content was lower t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochemistry. Biokhimiia

دوره 64 9  شماره 

صفحات  -

تاریخ انتشار 1999